TABLE 1.

Primers used in this study

PrimerNucleotide sequencea
GP2a-EcoRI-ForATATATGAATTCGCCACCATGAAATGGGGTCCATGC
GP2a-SphI-RevATATATGCATGCTCACCGTGAGTTCGAAGG
GP2a-NheI-ForATATGCTAGCGCCGCCACCATGAAATGGGGTCCATGC
GP2a-FLAG-EcoRI-RevATATGAATTCTCACTCGAGCTTGTCATCGTCGTCCTTGTAGTCCATCCGTGAGTTCGAAGGAAAAATTGC
GP3-EcoRI-ForATATAGAATTCGCCACCATGGCTAATAGCTGTGC
GP3-BamHI-RevTATATGGATCCCTATCGCCGCGCGGC
GP4-EcoRI-ForATATAGAATTCGCCACCATGGCTGCGCCCCTTC
GP4-BamHI-RevTATATGGATCCTCAAATTGCCAGTAAGATG
GP5-EcoRI-ForGCCGGAATTCGGAGCCGCCGCCACCATGTTGGGGAGATGCTTGAC
GP5-HindIII-XhoI-RevATCACTCGAGAAGCTTCTAAAGACGACCCCATTGTTC
CD163-HindIII-ForATATAAGCTTATGGACAAACTCAGAATGGTGCTAC
CD163-XhoI-RevATATCTCGAGTCATTGTACTTCAGAGTGGTCTCCTG
  • a Primer sequences are in the 5′ to 3′ direction. Restriction enzyme sites in the primers are underlined.