TABLE 1.

Details of ICP0 proteins expressed by viruses

VirusICP0 structurefDescription
17+a1-775Wild type
dl1403b1-105ICP0 null mutant
dl1403Rc1-775ICP0+ rescuant
FXEd1-105::150-775RING finger domain deletion mutant
FXERc1-775Rescuant with wild-type RING finger
K144E mutantePoint mutation at amino acid 144Mutant with substitution in RING finger helix
N151D mutantePoint mutation at amino acid 151Mutant with substitution in RING finger helix
  • a Parental virus HSV-1 Glasgow strain 17 syn+ (4).

  • b Has a 2-kb lesion in both inverted repeat copies of the ICP0 gene (54).

  • c Rescued virus was obtained by cotransfection with mutant virion DNA and a plasmid containing a fragment comprising the ICP0 gene (18, 50).

  • d Has a defined lesion in the ICP0 RING finger domain yielding a deletion of 45 amino acids (16-18, 24).

  • e Has a single amino acid substitution in the alpha helix of the ICP0 RING finger (15).

  • f Amino acids included or relevant mutation in ICP0 variant.