TABLE 4.

Relative neutralization of pseudotyped COT6.15 envelope MPER mutantsa

COT6.154E10BB34CAP206SAC21
IC50RatiobID50RatiocID50RatiocID50Ratioc
Wild type0.91.01,3921.01,2561.03171.0
A662G0.120.14,8990.32,4430.59780.3
L663A0.020.08,7140.27,9710.25,6600.1
D664A0.770.91,1491.28441.52381.3
S665A0.140.25,4950.31,5620.81,7870.2
W666A0.510.65,5540.34,2940.34460.7
K667A0.050.13,2610.41,6940.71,7340.2
N668A1.31.48311.74253.02081.5
L669A0.050.13,8470.43,1380.41,1950.3
W670A0.110.113210.51,0541.21053.0
S671A0.040.03,1020.41,6140.89280.3
W672A>25>252,9590.52,2440.64680.7
F673A>25>257791.84982.5<50>6.3
D674A1.41.6<50>25<50>25<50>6.3
D674S2.492.8<50>259014.0<50>6.3
D674N0.330.46632.16432.01492.1
I675A0.040.04,0690.32,0650.67180.4
T676A21.7724.22,3800.68951.45240.6
K677A0.050.14,6710.32,1510.611540.3
W678A0.050.13,8420.41,8850.71,0070.3
L679A0.090.12,0850.71,4480.92251.4
W680A10.8912.17311.99041.41422.2
  • a Cases with more than a 3-fold drop in the ID50 or IC50 are in boldface.

  • b (Mutant IC50)/(wild-type IC50) ratio.

  • c (Wild-type ID50)/(mutant ID50) ratio.