TABLE 1.

Epidemiologic data of six specimens from a child care center outbreak of acute gastroenteritisa

Sample IDSexAgeDate of (mo/day/yr):Symptom(s)Result for AstV-VA1 with:Result for othersc
OnsetSampleSanger sequencingPyrosequencingReal-time RT-PCR
AF43 yr8/19/088/19/08Diarrhea, vomiting, abdominal crampsNegNegWeak PosbNeg
BM10 mo8/18/088/18/08DiarrheaPosPosPosPos for human astrovirus
CF10 mo8/04/088/19/08DiarrheaPosPosPosNeg
DM2 yr8/19/088/19/08Diarrhea, vomitingNANegWeak PosbNeg
EM2 yr8/19/088/19/08Diarrhea, vomitingNegNAWeak PosbNeg
FM6 mo8/25/088/25/08DiarrheaNANAPosPos for human astrovirus
  • a ID, identification; Pos, positive; Neg, negative; F, female; M, male; NA, not applicable.

  • b Sample A showed positive results in late amplification cycles from one out of four separately extracted nucleic acid templates; samples D and E showed positive results in late amplification cycles from three out of four separately extracted nucleic acid templates.

  • c Other tests included those for the following: parasites; GI, GII, and GIV noroviruses; sapovirus and human astrovirus; rotavirus; and group F adenovirus.