TABLE 1.

Plasmids used in this study

PlasmidCharacteristic(s)Reference
pcDNA3.1(−)Eukaryotic expression vector carrying a CMV promoter and BGH polyadenylation signalInvitrogen
pIRES-EGFPSource of the EGFP geneClontech
pYS1190Source of the mCherry geneUnpublished
pTM-PolI-WSN-AllEight-unit plasmid for transcribing PB2, PB1, PA, NP, HA, NA, M, and NS vRNAs by means of HPI 16
pCAWS-NPEukaryotic expression of NP used as the helper plasmid 16
pYA3994Source of prokaryotic GFP cassetteLab collection
pYA4464Vector with p15A ori sequence and Cmr cassetteLab collection
pYA4749GFP expression vector with p15A ori constructed by fusing DNA segments from pYA3994 and pYA4464This study
pYA4337PB2 gene inserted into pcDNA3.1(−)This study
pYA4338PB1 gene inserted into pcDNA3.1(−)This study
pYA4339PA gene inserted into pcDNA3.1(−)This study
pYA4379CPI and MTI cloned into pcDNA3.1(−) to create a bidirectional vector to synthesize vRNA by means of CPI and mRNA by means of the CMV promoterThis study
pYA4383PB2 cDNA cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and vRNA by means of CPIThis study
pYA4384PB1 cDNA cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and vRNA by means of CPIThis study
pYA4385PA cDNA cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and vRNA by means of CPIThis study
pYA4386NP cDNA cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and vRNA by means of CPIThis study
pYA4387EGFP gene cloned into pYA4379 to synthesize mRNA by means of the CMV promoter and antisense RNA (vRNA-like) by means of CPIThis study
pYA4380CPI and MTI cloned into modified pcDNA3.1(−) to synthesize vRNAThis study
pYA4388HA cDNA inserted into the AarI sites in pYA4380 to synthesize vRNA by means of CPIThis study
pYA4389NA cDNA cloned into pYA4380 to synthesize vRNA by means of CPIThis study
pYA4390M cDNA cloned into pYA4380 to synthesize vRNA by means of CPIThis study
pYA4391NS cDNA cloned into pYA4380 to synthesize vRNA by means of CPIThis study
pYA4392EGFP gene cloned into pYA4380 to transcribe antisense RNA (vRNA-like) by means of CPIThis study
pYA4688CPI replaced with HPI in pYA4392 to transcribe the EGFP gene into antisense RNA (vRNA-like)This study
pYA4519Eight influenza cDNA cassettes cloned into one plasmid to synthesize vRNAs by means of CPI and PB2, PB1, PA, and NP mRNA by means of the CMV promoterThis study
pYA4731mCherry gene cloned between the CMV promoters and BGH terminator in pcDNA3.1(−)This study
pYA4732CMV-mCherry-BGH cassette from pYA4731 inserted into pYA4519 at the SrfI siteThis study