TABLE 4.

Mutagenesis of key residues in Fab Z13e1 that contact MPER peptide

MutationRegion% Apparent affinity relative to Z13e1
Peptidegp41
Wild type100.0100.0
LeuL92→AlaCDR L32.31.1
LeuL92→Ser/LeuL93→AspCDR L31.90.4
AsnH31→AlaCDR H18.915.6
TyrH33→AlaCDR H1<0.1<0.1
TyrH33→PheCDR H10.40.2
HisH50→GlnFR H23.53.6
HisH50→AlaFR H2<0.1<0.1
IleH52→AlaCDR H20.90.5
PheH100B→AspCDR H32.20.5
TyrH100G→AlaCDR H32.10.7