Virus | Plaque size^{a} [mm^{2}] (P value) | Burst size, [PFU/cell]^{b} (P value) | Virus yield^{c} [10^{5} PFU/ml] (P value) | Virion DNA copies/PFU^{d} (P value) |
---|---|---|---|---|

C700-A | 1.1 ± 0.09 | 30 ± 0.8 | 19 ± 2.8 | 100 ± 10 |

C700-B | 1.1 ± 0.10 | 26 ± 2.6 | ND^{e} | ND |

Q282R-A | 1.0 ± 0.09 | 19 ± 1.7 | 8.6 ± 3.0 | 230 ± 60 |

Q282R-B | 1.0 ± 0.09 (<0.01) | 23 ± 1.8 (<0.05) | 9.0 ± 3.8 (<0.01) | 320 ± 80 (<0.05) |

D270A/D271A-A | 0.93 ± 0.10 | 20 ± 2.8 | 11 ± 2.5 | 170 ± 2 |

D270A/D271A-B | 0.94 ± 0.10 (<0.01) | 20 ± 0.0 (<0.05) | 12 ± 0.7 (<0.05) | 170 ± 20 (>0.05) |

Q282R/D270A/D271A-A | 0.92 ± 0.09 | 19 ± 2.7 | 7.1 ± 1.5 | 400 ± 50 |

Q282R/D270A/D271A-B | 0.91 ± 0.07 (<0.01) | 18 ± 0.0 (<0.05) | 7.2 ± 4.2 (<0.01) | 400 ± 10 (<0.05) |

↵

*a*Plaque size is expressed as average and standard deviation for 20 plaques at 72 h postinfection. Student's*t*test was used to calculate the*P*value of the statistical significance of the differences between the mutant (both A and B) and the control viruses (C700-A and C700-B) expressing wild type UL42.↵

*b*Burst size was calculated by the ratio of peak titer (36 h postinfection) of the single growth curve assay over the number of cells. Data are averages and standard errors for two experiments. An ANOVA test was applied to calculate the*P*value.↵

*c*Virus yield was the peak titer of progeny virus harvested at 72 h after infection with an MOI of 0.01. Data are averages and standard errors for two experiments. An ANOVA test was applied to calculate the*P*value.↵

*d*Virion DNA copies/PFU and standard errors were calculated by determining the ratio of numbers of DNA copies present in cell-free medium over viral titers at 72 h postinfection with the inoculation of an MOI of 0.01. An ANOVA test was applied to calculate the*P*value.↵

*e*ND, not determined.