TABLE 3.

lacZ mutation frequency replicated by UL42 mutantsa

VirusNo. of total plaquesNo. of clear/light-blue plaquesMutation frequency [%] (P value)
C700-A7,7694/00.05
C700-B9,3056/00.06
Q282R-A10,20311/40.15 (0.078)
Q282R-B14,73711/80.13 (0.130)
D270A/D271A-A38,43370/90.21 (0.037)
D270A/D271A-B23,46450/40.23 (0.002)
D270A/D271A/Q282R-A26,51733/90.16 (0.033)
D270A/D271A/Q282R-B19,74821/140.18 (0.017)
• a The mutation frequency was calculated as the ratio of number of clear and light blue plaques to the number of total plaques. The P value was determined by using a chi-square test by comparing the mutation frequency of each mutant with those of both control viruses, C700-A and -B.