Genetic analysis of deferential growth in wild type and IFN-deficient MEFs using UK × RRV reassortant virusesa

ReassortantGeneVirus growth ratio
12345b67891011IFNR KO/wild typeSTAT1 KO/wild type
25-1-1RUUR R URURRU1.40.9
24-1-1RUUR R URURUU2.03.0
7-1-1RRRR R RRRRRU2.83.6
19-1-1RRRR R RRURRR3.71.0
14-1-1URUR R RRRRRR4.05.0
11-2-1-1RURR R URRRRU4.15.9
27-3-1URRR R RRRRRR4.73.6
25-2-1RRRR R URRRUR7.45.9
13-1-1RRRR R URRRRR7.77.5
22-1-1RRRR R RRURRR7.98.6
8-1-1RRUR R RRRRRU8.816.4
1-2-1RURR U RRRRRU133.3104.9
36-2-1UUUU U UUURUR141.0125.4
6-1-1RRRR U URURRR142.4135.6
27-2-1RRRR U URRRRR160.0135.8
4-1-1RRRR U URRRRU178.2214.3
36-1-1RURR U RRUURR221.8141.8
21-1-1UUUU U RRURRR230.0151.8
9-8-1UUUU U URUUUU271.4256.7
27-1-1-1UURU U RURUUU287.6139.8
32-2-1-1UURU U URUUUR393.8358.1
20-1-1URUU U RUUURR582.1410.3
  • a R represents gene from RRV, and U represents gene from UK. Wild-type, IFNR KO, and STAT1 KO MEFs were infected with UK, RRV, or the indicated UK × RRV reassortant viruses at an MOI of 1 for 24 h. Virus yields were determined by focus-forming assay on MA104 cells. The virus growth ratio was calculated as follows: yield of IFNR KO or STAT1 KO MEF (FFU/ml)/yield of wild-type MEF (FFU/ml). The ratios presented are the means of quadruple data points. Boldface type highlights gene 5 segregation.

  • b Cosegregation of gene 5 with a distinct UK or RRV growth phenotype in MEFs is significantly different from random distribution (P < 0.001 by the permutation method of Brown and Fears [8]).