TABLE 2.

Quantitative PCRs for the 5′ UTR and E2 regions of HCVa

Region for quantificationNo. of copies/ml5′ UTR/E2 ratio
5′ UTRE2
R22.0 × 1061.7 × 1061.17
R45.3 × 1062.2 × 1062.44
T5NDbNDb
K18.3 × 1058.4 × 1050.99
K23.6 × 1053.5 × 1051.01
K38.6 × 1055.1 × 1051.7
K4pre8.1 × 1053.3 × 1052.45
K4af244.5 × 1054.4 × 1051.01
  • a For quantification of the 5′ UTR and E2 regions, the TaqMan Fast PCR Universal mixture and the 7500 Fast Real-Time PCR system (Applied Biosystems) were used in a two-step method with the primers and probes shown in Table 1 according to the manufacturer's protocol. The copy number of HCV was determined by the standard-curve method with serial dilutions of the synthesized full-length HCV RNA.

  • b ND, not determined due to sample shortage.