TABLE 2.

Primary antibodies used for immunofluorescence and immunoblotting

AntigenaAntibodySourceTypebDilution
ImmunofluorescencecImmunoblottingd
UL1385484, customOpen BiosystemsR1:2501:1,000
Myc epitopeClone 9E10MilliporeM1:15001:5,000
Myc epitope71D10Cell SignalingR1:200NDe
MHC-I, HLA-B/CHC10GiftfMND1:50
IE1/23H4GiftgMND1:100
pp712H10-9GiftgMND1:100
α-TubulinDM 1ASigmaMND1:12,000
β-ActinA2103SigmaMND1:1,000
Golgi GM130Clone 35BD Transduction LaboratoriesM1:100ND
Golgi G58KClone 58K-9Gene TexM1:125ND
GS 27Clone 25BD Transduction LaboratoriesM1:100ND
PDIRL 77Affinity BioreagentsM1:50ND
EEA114/EEA1BD Transduction LaboratoriesM1:250ND
  • a GM, Golgi matrix; GS, Golgi SNARE.

  • b R, rabbit; M, mouse monoclonal.

  • c Dilution in PBS supplemented with BSA and Tween 20; incubated for 60 to 90 min at room temperature or at 4°C overnight in the case of the rabbit anti-Myc antibody.

  • d Dilution in TBS supplemented with BSA and Tween 20; incubated for 60 to 90 min at room temperature.

  • e ND, not done.

  • f Provided by Lonnie Lybarger (51).

  • g Generous gift from Tom Shenk, Princeton University.