TABLE 1.

Sequences and positions of primers used for UDPS of HBV

PrimeraSequence (5′→3′)RT position
1 FWCCTGCTGGTGGCTCCAGTT−34-53
1 RWAGAGAAGTCCACCACGAG124-140
2 FWCCTGCTCGTGTTACAGGCG58-72
2 RWCCGCAGACACATCCAGCG242-259
3 FWCCGTGTGTCTTGGCCAAA162-179
3 RWGACAAACGGGCAACATAC330-347
4 FWGCTGCTATGCCTCATCTTCT286-305
4 RWGAYGATGGGATGGGAATAC471-489
5 FWGCACGACTCCTGCTCAAGG396-414
5 RWCCCKACGAACCACTGAACAA561-580
6 FWGTATTCCCATCCCATCRTC471-489
6 RWCGGTAWAAAGGGACTCAMG649-667
7 FWTTGTTCAGTGGTTCGTMGGG561-580
7 RWGGGTTAAATGTATACCCAVAG689-710
8 FWCKTGAGTCCCTTTWTACCG649-667
8 RWCKTGAGTCCCTTTWTACCG649-667
  • a The primers included 5′ extensions (left GCCTCCCTCGCGCCATCAG and right GCCTTGCCAGCCCGCTCAG adaptors not shown in the table) which provide binding sites for pyrosequencing.