TABLE 1.

Summary of EBV gB linker insertion mutantsa

Mutant nomenclatureSite(s) of mutation (aa)bInsertion sequencec
gBNoneNone
gB3030-31AP-CLNTP-PA
gB8888-89DN-CLNNN-II
gB115115-116AD-LFKQD-SV
gB190190-191DA-LFKHA-PG
gB198198-199TY-CLNTY-RT
gB208208-209CL-MFKHL-IT
gB236236-237FY-CLNIY-DG
gB263263-264YD-MFKHD-NR
gB307307-308TI-CLNTI-AT
gB320320-321TD-CLNTD-EG
gB324324-325TS-CLNTS-SF
gB353353-356HE-CLNNE-KY
gB361361-362DR-LFKHR-YT
gB380380-381LL-VFKQL-AW
gB421421-422GS-MFKQS-TP
gB486486-487LE-CLNME-QK
gB561561-562FS-CLNIS-FI
gB597597-598YF-LFKHF-QS
gB602602-603EI-LFKQI-HV
gB620620-621IA-CLNIA-TL
gB632632-633SL-MFKHL-IE
gB646646-647SR-CLNTR-DE
gB675675-676LR-MFKQR-KD
gB703703-704SV-FKHSV-GQ
gB717717-718GL-LFKHL-FS
gB734734-735FG-CLNTG-GM
gB802801-802AL-LFKQL-HE
gB832832-833RF-LFKHF-PG
gBΔ798Truncated at 798Noned
  • a The nomenclature of mutants used throughout the paper was determined by the first amino acid prior to the site of linker insertion. All mutants were sequenced at the Northwestern University sequencing facility, and the amino acid composition of the insertion was confirmed.

  • b aa, amino acids.

  • c The 2 amino acids before and after the hyphens are the wild-type EBV gB sequence that flanks the linker insertion. A unique PmeI restriction site was introduced into all gB linker insertion mutants and was used for diagnostic purposes.

  • d The gBΔ798 mutant possesses a unique BclI restriction site.