TABLE 1.

Results of mutations in the NwV coat protein

Mutation(s)AssemblyLCCProteolytic kineticsaResidual pKab
ProcapsidCapsid
Glu73GlnYesNative-likeAberrant(Glu73) 2.44-5.24(Glu73) 1.74-3.92
Asp124AsnNo(Asp124) 2.4-4.44(Asp124) 1.98-8.10
Glu278GlnYesNative-likeAberrant(Glu278) 4.5(Glu278) 6.27-6.73
Glu442GlnYesNative-likeNative-like(Glu442) 5.89-6.40(Glu442) 8.03-9.58
Asp87Asn/Asp124AsnNo(Asp87) 2.22-9.31(Asp87) 6.49-12.84
  • a See Fig. 7.

  • b A complete list of the pKa values for all ionizable residues is available from the authors.