TABLE 3.

Locations of EBV gB linker insertions in the predicted EBV gB structure

Linker siteLocation in predicted EBV secondary structureaLocation in predicted HSV-1 secondary structurebLocation in HSV-1 crystal structurec
gB30LLNA
gB88LE/Lβ3
gB115EE/Hβ5
gB190LLUS
gB198EEβ11
gB208EEβ11
gB236LE/LUS
gB263LLUS
gB307HHβ18
gB320ELUS
gB324EEβ20
gB353HHαB
gB361USE/Lβ21
gB380EEUS
gB421LLUS
gB486H/USH/LαC
gB561EE/Lβ29
gB597EEβ33
gB602EEβ34
gB620EEβ35
gB632E/USLUS
gB646USL/HαE
gB675HHNA
gB703HUSNA
gB717EUSNA
gB734HUSNA
gB802HHNA
gB832H/LHNA
  • a The PredictProtein program (http://www.predictprotein.org) was used for analysis of EBV gB, and the PHD neural network was utilized to predict gB secondary structure. E, β-sheet; H, α-helix; L, loop; US, unstructured.

  • b The PHD neural network method was also employed to estimate the secondary structure of HSV-1 as previously described (53). E, β-sheet; H, α-helix; L, loop; US, unstructured.

  • c The results of secondary structure predictions were also compared to the HSV-1 gB crystal structure to assess the accuracy of the predictions (26). NA, not applicable; this region was not determined in the HSV-1 gB crystal structure.