TABLE 2.

Statistical analysis of the frequency of mutations in the KF9 epitope

Study subjectMean no. of amino acid differences between provirus and plasma virus GagaPredicted no. of substitutions:Observed no. of substitutions in KF9dP value
Per amino acidbIn KF9c
ES25.8070.0400.3601.875<0.001
ES311.00.0790.7113.000<0.001
ES84.1920.0300.2702.692<0.001
ES74.9290.0350.31500.899
ES93.4290.0250.22100.956
  • a Mean numbers of amino acid differences between proviral and plasma Nef were calculated to include mutations that were present in <100% of the plasma or proviral Nef quasipecies.

  • b The predicted numbers of substitutions per amino acid were determined by dividing the total number of amino acid differences by the number of amino acids (140) in the region of Nef that was analyzed.

  • c The predicted numbers of substitutions in epitope KF9 were determined by multiplying the predicted number of substitutions per amino acid for Nef by the number of amino acids in this epitope (9).

  • d The observed numbers of substitutions in KF9 were calculated to include mutations that were present in this epitope in <100% of the plasma or proviral Nef quasipecies.