TABLE 1.

Nef peptides targeted by PBMC from nine HLA-B*57-positive ES

SubjectPeptide sequenceaNef amino acid coordinatesSFC/million PBMCb
ES2YKAAVDLSHFLKEKG81-95605
ES3WVYHTQGYFPDWQNY113-127145
KRQDILDLWVYHTQG105-119135
ES5QEEEEVGFPVRPQVP61-75145
PGPGIRYPLTFGWCF129-144230
EREVLEWKFDSRLAF177-191165
LAFHHMARELHPEYY188-203230
ES6MGGKWSKRSVVGWPT1-151,650
RRAEPAADGVGAVSR21-35600
PAADGVGAVSRDLEK25-391,020
SSNTAANNADCAWLE45-59210
ILDLWVYHTQGYFPD109-12380
ES7WVYHTQGYFPDWQNY113-127400
ES8No response
ES9QEEEEVGFPVRPQVP61-7575
YKAAVDLSHFLKEKG81-95410
TQGYFPDWQNYTPGP117-13175
ES19YKAAVDLSHFLKEKG81-95460
PGPGIRYPLTFGWCF129-143210
ES23QVPLRPMTYKAAVDL73-87190
YKAAVDLSHFLKEKG81-95120
WVYHTQGYFPDWQNY113-1271,905
LAFHHMARELHPEYY189-203260
  • a Peptides in bold are the optimal HLA-B*57-restricted epitopes. These optimal peptides were used to confirm that the responses elicited by the overlapping peptides were in fact to HLA-B*57-restricted epitopes. In the case of ES2, there were not sufficient PBMC available to confirm that the response elicited by Nef 81-95 was targeted to the optimal epitope Nef 82-90.

  • b The responses are shown as IFN-γ SFC per million PBMC.