TABLE 1.

IE gene expression in ATRX-depleted cellsa

CellsVirus(es)No. of cellsb
High IELow IETotalIE positive (%)
HF-shLuciSub825667799.1
HF-shA90Sub82783676614.8
HF-shLuciSub82 + AD169UV94638326.1
HF-shA90Sub82 + AD169UV113943927.8
HF-shLuciAD16969615847.5
HF-shA90AD16954616935.5
  • a HF-shLuci or HF-shA90 cultures were infected with ADsubUL82 (Sub82; 0.2 infectious units/cell), ADsubUL82 plus UV-irradiated HCMV (AD169UV; MOI of 3, based on original titer), or wild-type HCMV (AD169; MOI of 0.3). At 12 h postinfection, coverslips were fixed and reacted with antibodies specific for HCMV IE proteins and ATRX.

  • b IE-positive cells were classified as present in low or high amounts, as shown in Fig. 9A and B, respectively.