TABLE 2.

Set of identified influenza A virus-derived HLA class I- and class II-restricted T-cell epitopes

SequenceaProteinPositionSupertypeConservancy (%)bNo. testedNo. of respondersRecognition frequency (%)cMax SFC/106No. of alleles boundd
ALASCMGLIYM1124-133A1100172121301
TFEFTSFFYPB1489-497A1100173181223
VSDGGPNLY*PB1591-599A196173181061
GILGFVFTL*M159-67A21002110483343
GLIYNRMGAM1130-138A26521314941
ILGFVFTLM160-67A2100217333084
ILSPLTKGILM152-61A210021419822
QIAILVTTVNA28-36A23521151012
QLVWMACHSAANP329-339A265212102271
TTYQRTRAL*NP148-156A25221210551
ALQLLLEVNS2102-109A230214191911
ITFMQALQLLNS2107-116A28721210751
QLMWALGENMAPA365-375A217212101121
RTMAWTVVNSIPA86-96A274215242302
FNMLSTVLGVPB1412-421A2100215241134
FSMELPSFGVPB1505-514A210021210715
FVANFSMELPB1501-509A210021210775
FVEALARSIPB1254-262A22221210902
LLFLKVPAPB17-14A296213142083
MMMGMFNMLPB1407-415A2100213142125
KTRPILSPLTKM148-58A310022298282
RMVLASTTAK*M1179-188A31002215742
RMVLSAFDERNP67-76A3912215661
TLARSICEKPB1257-265A3742215612
SFSFGGFTFKPB2327-336A39622291275
LYRKLKREITFM1100-110A24781417952
SWPDGAELPFNA456-465A243914321951
LPSFGVSGIPB1509-517B78723141111
AEIEDLIFLANP251-260B446527271685
FEDLRVSSFNP338-346B4461274153392
YERMCNILKGNP221-230B4410027272121
AEKPKFLPDLYPA102-112B446527271481
AESRKLLLIPA660-668B444827271044
CELTDSSWIPA415-423B443927311813
AEIMKICSTPB1741-749B44302714732
CEKLEQSGLPB1263-271B441002714883
FEFTSFFYPB1490-497B441002714753
FESKSMKLPB1357-364B447027311781
PRYVKQNTLKLATGMHA325-339DR173027788
KGILGFVFTLTVPSEM158-72DR100304131679
MGAVTTEVAFGLVCAM1136-150DR483013361
RMVLASTTAKAMEQMM1179-193DR1003013439
GDVFVIREPFISCSHNA112-126DR43304131926
GKIVHISPLSGSAQHNA263-277DR3030135811
PTIIERNITEIVYLTNA57-71DR133013537
LVWMACHSAAFEDLRNP330-344DR6530310485
RSKFLLMDALKLSIEPA281-295DR8330134610
MAFLEESHPGIFENSPB192-106DR7430310455
MDVNPTLLFLKVPAQPB11-15DR9630620349
MMGMFNMLSTVLGVSPB1408-422DR1003031014810
RMFLAMITYITRNQPPB1316-330DR353013367
SPGMMMGMFNMLSTVPB1404-418DR10030413567
LGTFDTVQIIKLLPFPB2607-621DR303013596
MRILVRGNSPAFNYNPB2645-659DR173027598
  • a *, epitopes that have been identified in previous studies.

  • b Conservancy indicates the fraction of the 23 influenza A virus strains included in this study that contain the exact peptidic sequence.

  • c Recognition frequency indicates the fraction of the tested donors responding to each epitope.

  • d Each peptide was tested for binding to the common HLA alleles that belong to the corresponding supertype based on binding predictions. The number of alleles tested (for supertype) was 4 (A1), 5 (A2), 5 (A3), 2 (A24), 5 (B7), 6 (B44), and 11 (DR).