TABLE 1.

Genotypes of reassortant viruses between Prague (H7N7) and Yokohama (H3N2) virusesa

GenotypeGene originbFrequency (no. of viruses)
PB2PB1PAHANPNAMNS
A1YYPYPYPY6
A2YYPYPYYY5
A3YYPYPPPP4
A4YYYYPYYY11
A5YYYPPYPY1
A6YYYYPYPY23
A7YYYYPPPP6
A8YYYYPPYY2
A9YYPYPYPP4
A10YYYYPYPP14
A11YYPYPYYP1
A12YYYYYPYY3
A13YYYYPPPY14
A14YYPYYYPP1
A15YPPYPPYY2
A16YPPYPYPY1
A17YYYYYPYP1
A18YYYPPPPY1
A19YYYYYPPY1
A20YYYYYYPP2
A21YPPYPYPP1
A22YYYYPYYP1
A23YYPYPPPY4
A24YYYYYYYY4
A25YYYYYPPP1
A26YYYYYYPY1
A27YPPYPPPY3
A28YYPYPPYY1
A29YPPYYPPP1
No. of viruses possessing the respective Yokohama segment/total no. of viruses120/120112/12086/120118/12015/12076/12031/12083/120
  • a 293T cells were transfected with plasmids for the generation of vRNAs for both the Prague and Yokohama viruses together with the four protein expression plasmids of WSN. The cotransfection supernatants were plaque purified on MDCK cells. The genotypes of the plaque-purified viruses were determined by using strain-specific PCRs with identifying primers.

  • b P, Prague; Y, Yokohama.