TABLE 2.

Primers used for markerless mutagenesis of TB40-BAC4-UL128

Mutant BACPrimer directionPrimer sequencea
TB40-BAC4-UL128ccta18-21ForwardCTGACGCCGTTCTTGACAGCGTTGTGGCTGTTATTGGCAGCAAGCGCAGTGCCACGGGTACGCGaggatgacgacgataagt
ReverseGAATTCGCAACATTCTTCTGCGCGTACCCGTGGCACTGCGCTTGCTGCCAATAACAGCCACAACcaaccaattaaccaattctga
TB40-BAC4-UL128ccta38-42ForwardCGCGCAGAAGAATGTTGCGAATTCATAAACGTCAACGCACCGCCGGCAGCATGTTACGATTTCAAAAaggatgacgacgataagt
ReverseGACGGTGAAGCGATTGCACATTTTGAAATCGTAACATGCTGCCGGCGGTGCGTTGACGTTTATGAATcaaccaattaaccaattctga
TB40-BAC4-UL128ccta60-62ForwardAGTAGATTTTCATGAATACACAGGCTGCGGTGTCCGGCAGGCGCAGTCTGCTACAGTCCCGaggatgacgacgataagt
ReverseGCGAATCTCAGCCGTTTTCTCGGGACTGTAGCAGACTGCGCCTGCCGGACACCGCAGCCTGcaaccaattaaccaattctga
TB40-BAC4-UL128ccta72-74ForwardCGGACGGCGAAGTCTGCTACAGTCCCGAGAAAACGGCTGCGATTGCTGGGATCGTCACCACCATGaggatgacgacgataagt
ReverseTGGCGTGTCAATGAATGGGTCATGGTGGTGACGATCCCAGCAATCGCAGCCGTTTTCTCGGGACTcaaccaattaaccaattctga
TB40-BAC4-UL128ccta82-86ForwardACGGCTGAGATTCGCGGGATCGTCACCACCATGACCGCTTCATTGACAGCACAGGTCGTACACAACAaggatgacgacgataagt
ReverseGTAGTTGCAGCTCGTCAGTTTGTTGTGTACGACCTGTGCTGTCAATGAAGCGGTCATGGTGGTGACGcaaccaattaaccaattctga
TB40-BAC4-UL128ccta104-108ForwardTTTTGTACCCCCCTTCCGTGTTGTAGGTTATACCTCGCAGCTGCAGGGGCAATACGCTGCGGCAAAGaggatgacgacgataagt
ReverseGTACTGCGCCTTGTCGTTCACTTTGCCGCAGCGTATTGCCCCTGCAGCTGCGAGGTATAACCTACAAcaaccaattaaccaattctga
TB40-BAC4-UL128ccta136-139ForwardGCCGCTGGCAGCGTTCCCTATCGATGGATCAACCTGGCATACGCAGCAATAACCCGGATCGTGGaggatgacgacgataagt
ReverseTCTCCAGGTACTGATCCAGGCCCACGATCCGGGTTATTGCTGCGTATGCCAGGTTGATCCATCGAcaaccaattaaccaattctga
TB40-BAC4-UL128ccta154-158ForwardCGGATCGTGGGCCTGGATCAGTACCTGGAGAGCGTTGCGGCAGCTGCAGCACTGGATGTGTGCCGCGaggatgacgacgataagt
ReverseCAGCATATAGCCCATTTTAGCGCGGCACACATCCAGTGCTGCAGCTGCCGCAACGCTCTCCAGGTACcaaccaattaaccaattctga
TB40-BAC4-UL128ccta155-157ForwardCGGATCGTGGGCCTGGATCAGTACCTGGAGAGCGTTAAGGCAGCCGCACGGCTGGATGTGTGCCGCGCaggatgacgacgataagt
ReverseCAGCATATAGCCCATTTTAGCGCGGCACACATCCAGCCGTGCGGCTGCCTTAACGCTCTCCAGGTACTcaaccaattaaccaattctga
TB40-BAC4-UL128ccta163-165ForwardCCTGGAGAGCGTTAAGAAACACAAACGGCTGGATGTGTGCGCAGCTGCAATGGGCTATATGCTGCAGTGaggatgacgacgataagt
ReverseCAAACACACATTTTATTATTCACTGCAGCATATAGCCCATTGCAGCTGCGCACACATCCAGCCGTTTGTcaaccaattaaccaattctga
  • a Uppercase, HCMV homology; lowercase, homology to template plasmid pEP-Kan-S; bold, sequence of interest.