TABLE 1.

Anti-VP6 VHH neutralizing concentration for different RV strains

BinderNeutralizing VHH concna (μg/ml)
INDC486B223WaH2
Monovalent
    2KA4NegNegNegNegNeg
    2KD10.980.980.980.203.90
    3A61.951.957.800.9815.60
    3B21.951.950.980.203.90
Unrelated VHHNegNegNegNegNeg
Bivalent
    Biv2KA4NegNegNegNegNeg
    Biv2KD115.603.9062.503.9015.60
    Biv3A615.603.90Neg3.9015.60
  • a That is, the minimum VHH concentration that reduces >80% of FFU generated by 100 FFU of each RV strain. Strains: BRV IND (SbI, P[5]G6), BRV C486 (SbI, P[1]G6), BRV B223 (SbI, P[11]G10), human RV Wa (SbII, P1A[8]G1), equine RV H2 (Sb no I no II; P[12]G3). Neg, no neutralizing activity was observed at the highest concentration tested (62.50 μg/ml).