TABLE 1.

Viral content of extracellular virions

Protein group (expected in virions)aProteinMWbSize (aa)cNo. of unique peptidesd% Coveragee% of totalfTotal no. of proteins
Capsid
    UL6 (+)74.167612.5
    UL15 (?)Terminase80.9735NA
    UL17 (+)74.67031023.8
    UL18 (+)VP2334.33181565.7
    UL19 (+)VP5/ICP5149.11,3745459.5
    UL25 (+)62.7580819.5
    UL26 proteaseVP24 (+)26.6247741.3
    VP21 (−)37.3363NA
    COOH termini (−)2.625NA
    UL26.5VP22a (−)31.2304NA
    COOH termini (−)2.625NA
    UL28 (?)ICP18.585.6785NA
    UL33 (?)14.4130NA
    UL35 (+)VP2612.1112339.3
    UL38 (+)VP19C50.3465923.9
    Total10726.78
Envelope
    UL1 (+)gL24.9224424.6
    UL10 (+)gM51.4473211.2
    UL20 (+)24.2222NA
    UL22 (+)gH90.48381118.7
    UL27 (+)gB100.39042230.2
    UL34 (−)29.8275NA
    UL43 (?)44.9434NA
    UL44 (+)gC55.0511726.6
    UL45 (+)18.2172539.0
    UL49A (+)gN9.291NA
    UL53 (?)gK37.6338NA
    UL56 (+)21.2197222.8
    US4 (+)gG25.223823.8
    US5 (?)gJ9.692NA
    US6 (+)gD43.3394723.2
    US7 (+)gI41.4390416.7
    US8 (+)gE59.1550918.4
    US8A (?)16.8159NA
    US9 (+)10.090137.8
    Total761912g
Tegument
    RL1 (+)ICP34.526.224813.4
    RL2 (?)ICP078.57761021.3
    RS1 (?)ICP4132.81,2982022.6
    UL4 (−)78.2718NA
    UL7 (?)33.1296425.0
    UL11 (+)10.596220.8
    UL13 (+)57.2518310.0
    UL14 (+)23.9219110.5
    UL16 (+)40.4373732.4
    UL21 (+)57.6535927.5
    UL23 (?)TK41.037614.4
    UL31 (−)34.0306NA
    UL36 (+)Large tegument335.93,1644220.7
    UL37 (+)ICP32120.61,1232233.2
    UL41 (+)Vhs54.9489930.1
    UL46 (+)VP11/1278.27181634.3
    UL47 (+)VP13/1473.86932551.2
    UL48 (+)VP16/ICP2554.34901545.5
    UL49 (+)VP2232.3301839.5
    UL50 (?)dUTPase39.1371626.7
    UL51 (+)25.5244427.0
    UL55 (?)20.5186437.6
    US2 (+)32.5291515.6
    US3 (+)52.848116.7
    US10 (+)34.1312313.7
    US11 (+)17.8161NA
    Total21854.423
Other
    UL2 (−)36.3334NA
    UL3 (−)25.6235NA
    UL5 (−)98.7882NA
    UL8 (−)79.9750NA
    UL9 (−)94.3851NA
    UL12 (−)67.5626NA
    UL24 (?)29.5269NA
    UL29 (−)ICP8128.41,196NA
    UL30 (−)136.41,235NA
    UL32 (−)64.0596NA
    UL39 (−)ICP6/10124.11,137NA
    UL40 (−)38.0340NA
    UL42 (−)51.2488NA
    UL52 (−)114.41,058NA
    UL54 (−)ICP2755.3512NA
    US1 (−)ICP2246.5420NA
    US12 (−)ICP479.888NA
  • a Localization is based on that described in references 2 and 56. +, expected; −, not expected; ?, uncertain.

  • b MW, estimated molecular weight (103).

  • c aa, amino acids.

  • d −, no peptides found.

  • e Values shown are the percentages of coverage of proteins by peptides. NA, not applicable.

  • f Relative peptide abundance.

  • g Thirteen proteins if UL20 is included (Table 2, MRM data).