TABLE 3.

Potential cellular proteins associated with extracellular virions identified by mass spectrometry

Protein nameMWaSize (aa)bNumber of unique peptides% Coveragec
14-3-3 protein28.2h2481d4.0
14-3-3 protein epsilon29.2255316.9
14-3-3 protein gamma28.324715.7
14-3-3 protein zeta/delta27.7245212.7
Actin41.8h3753e16.3
Annexin A138.734614.6
Annexin A238.6339313.6
Annexin A535.932015.0
Arf1/Arf 320.71813f26.0
Arf1/Arf3/Arf520.61812f11.0
Arf1/Arf3/Arf4/Arf520.61812f16.0
Arf420.5180110.0
ATP-dependent RNA helicase DDX3X73.266223.3
Casein kinase 245.139114.6
Cofilin 118.516617.4
Cyclophilin A18.0164864.8
Cystein-glycin-rich protein 120.6193111.4
Eukaryotic translation initiation factor 4H (eIF4H)27.4248428.2
GAPDHi36.133514.2
Growth factor receptor bound protein 225.221716.5
HSP7070.464137.8
Keratin 166.064412.5
Keratin 1059.559312.0
Macrophage migration inhibitory factor12.511519.5
Membrane attack complex inhibition factor (CD59)14.212819.4
Nucleoside diphosphate kinase A/B17.3152111.8
Peroxiredoxin-122.1199210.6
Peroxiredoxin-221.919815.5
Profilin-115.1140428.6
Programmed cell death protein 621.9191210.5
Rab24.0h2153g15.3
Rab2A/Rab 2B/Rab4B23.821416.1
Rab523.621515.1
Rab625.2223321.1
Rab7A23.520716.8
Rab1022.520015.5
Rab1124.5217211.1
Rab1524.421215.2
Rab33B25.722914.8
Rab3523.020115.5
Rab-like protein 326.423614.7
S100 calcium protein binding A1111.7105225.7
Sec14-like protein 446.640612.2
Tetraspanin 1322.2204110.3
Transferrin receptor protein 1 (CD71)84.976011.2
Translocase of inner mitochondrial membrane 50 (TIMM50)39.635313.1
Triosephosphatase isomerase26.7249111.7
Ubiquitin C8.676232.9
Ubiquitin-conjugating enzyme E2 L317.9154114.3
Total peptides85
  • a MW, estimated molecular weight (103).

  • b aa, amino acids.

  • c Percentage of coverage of protein by polypeptides detected by tandem mass spectrometry.

  • d Shared among the 14-3-3 protein family members.

  • e Shared among the actin family members.

  • f Shared among the indicated Arf proteins.

  • g Shared among the Rab protein family members.

  • h MW was averaged for these family members.

  • i GAPDH, glyceraldehyde-3-phosphate dehydrogenase.