TABLE 1.

Capacity of HCV-specific HLA-A2.1-restricted peptide epitope to bind to purified recombinant HLA-A2.1 molecules and to induce specific CTL or IFN-γ-producing T cells after direct injection

GeneResidue (aa)EpitopeBinding (% inhibition)aELISPOT (no. of specific spots/106 cells)bSpecific lysis (%) (E/T = 33)c
SequenceNameMcM1M2M3McM1M2M3
C35-44YLLPRRGPRLC35-445812731607325232
132-140DLMGYIPLVC132-1407102112062844441926
E1220-227IMHTPGCVE1 220-227550055012014
257-266TIRRHVDLLVE1 257-2660NDNDNDNDNDNDNDND
363-372SMVGNWAKVE1 363-372160172160328047366
E2401-411SLVSWLSQGPSE2 401-41130000000
614-622RLWHYPCTIE2 614-622920824720010342622
686-694ALSTGLIHLE2 686-69410004032601122651079
725-734FLLLADARVE2 725-7346603332156220833436
NS31073-1081CVNGVCWTVNS3 1073-108118054713227880
1169-1177LLCPSGHVVNS3 1169-1177772137322160945753
1406-1415KLTGLGLNAVNS3 1406-141571018845823018331540
1585-1593YLVAYQATVNS3 1585-159376001731901171916
NS4B1769-1777HMWNFITGINS4B 1769-17773001629330013350
1789-1797SLMAFTASINS4B 1789-179763716115717720311538
1807-1816LLFNILGGWVNS4B 1807-181694060522658019120
1851-1859ILAGYGAGVNS4B 1851-18593205206000
NS5A2221-2231SPDADLIEANLNS5A 2221-22313742102084022
2252-2260ILDSFDPLRNS5A 2252-2260340370308038
NS5B2578-2587RLIVFPDLGVNS5B 2578-25871004923411630512413346
2594-2602ALYDVVSTLNS5B 2594-26028039261331326081442
2727-2735KLQDCTMLVNS5B 2727-2735992525527729713385260
  • a Percent inhibition of binding of the FLP-control peptide onto purified HLA.A2.1 molecules for a ratio competitor peptide/control peptide = 30.

  • b ND, not done. Mc, control mouse; M1, M2, and M3, immunized mice.

  • c Percent specific lysis at an E:T ratio of 1:33.