TABLE 3.

Intracellular virus neutralization by anti-H IgAa

Cells and antibody (100 μg/ml)Apical supernatantCell lysate
Virus titer (10−4 PFU/ ml)% Reduction of virusbVirus titer (10−5 PFU/ ml)% Reduction of virusb
pIgR+
    Anti-H IgA0.09 ± 0.02d970.49 ± 0.05d91
    Anti-H IgG3.09 ± 0.32e115.82 ± 0.36e0
    CMc1.72 ± 0.21d515.71 ± 0.19e0
    None3.48 ± 0.3405.68 ± 0.390
pIgR
    Anti-H IgA2.85 ± 0.13d165.63 ± 0.24e9
    None3.41 ± 0.1806.16 ± 0.250
  • a Experiments were repeated on at least three occasions with similar results. Results of a representative experiment are shown. Data are means ± standard deviations (n = 3).

  • b Compared to no-antibody control.

  • c Conditioned medium (see the text).

  • d Mean virus titers were significantly lower (P < 0.001) than those from cells exposed to no antibody.

  • e Mean virus titers were not significantly lower (P ≥ 0.05) than those from cells exposed to no antibody.