TABLE 1.

Primers for NS5A sequencing

PartPrimerNameNucleotide sequence (5′-3′)Positiona
1External sense12sTCCCCCACGCACTATGTGCC6117
1External antisense12aTCAGTGGTCATGCCCGT6636
1Internal sense11sCAGTGGATTAATGAGGA6213
1Internal antisense11aTAGTGGAAATCCCCCAC6615
2External sense3sGGCATGGAACATTCCCCAT6496
2External antisense4aACGGATATTTCCCTCTCATCC7138
2Internal sense13sTACACCACGGGCCCCTG6522
2Internal antisense2aCCGAAGCGGATCGAAAGAGTCCA7087
3External sense4sACCCCTCCCACATTACAGCAG6859
3External antisense8aCCAAAGGTCTTAGTAGC7371
3Internal sense18sAACCTCCTGTGGCGGCA7017
3Internal antisense10aAGCTCCGCCAAGGCAGAAGA7350
4External sense17sGACTACAACCCTCCACT7203
4External antisense15aGGTGACGCAGCAAAGAGTTG7667
4Internal sense16sGTGGTGCACGGGTGCCCATTGCC7257
4Internal antisense14aCCTCCGCAGCGCATGGC7622
  • a Nucleotide positions indicate the carboxy-terminal ends of the primers within the HCV gene according to HCV-J (18).