TABLE 2.

Growth of NCO cells in soft agara

Cell lineNo. of colonies at:
Passage 2Passage 6
NCO0/0/0/00/0/0/0
NCO/v0/0/0/00/0/0/0
NCO/t60/55/50/71>200
NCO/wt-Akt0/1/0/20/1/3/0
NCO/ca-Akt0/1/0/00/0/0/0
NCO/hTERT1/0/1/00/0/0/0
NCO/wt-Akt+hTERT0/0/0/00/1/0/0
NCO/ca-Akt+hTERT0/1/0/11/0/0/0
  • a Petri dishes (35 mm) initially were seeded with 2 × 10 3 cells. Experiments were performed twice, each time in duplicate. Colonies were counted after 4 weeks.