TABLE 4.

Microsatellite analysis of plasma samples with heterogeneous viral sequencesa

LocusAllele(s) identified in sample obtained (mo/day/yr) from indicated subject
ZM229MZM215FZM247FZM246FZM246Mc 04/04/03
07/17/0210/19/0207/04/0210/19/0210/28/0311/01/0301/14/0304/04/03b
D8S117914, 1614, 1613, 1513, 1515, 1715, 17141414
D211S1130.2, 31.230.2, 31.228, 3628, 3628.2, 2728.2, 2728, 3127, 28, 3127, 28
D7S82011, 1211, 128, 108, 1010, 1110, 1110, 1310, 12, 1312
CSF1PO7, 127, 1210, 1110, 1110, 1210, 127, 107, 10, 1212
D3S135815, 1715, 17171716, 1716, 171515, 16, 1716, 17
TH016, 86, 87, 87, 87, 9.37, 9.37, 87, 8, 98, 9
D13S31712, 1312, 1310,1210, 1212, 1312, 1311, 1211, 12, 1411, 14
D16S53912, 1312, 139, 129, 1210101110, 11, 1210, 12
D2S133822, 2322, 2321, 2221, 22202019, 2219, 22, 2319, 23
D19S43312, 1412, 1411, 1311, 1314, 16.214, 16.213, 1413, 14, 1513, 15
vWA13, 2013, 2016, 1716, 171818151515
TPOX9, 119, 1110, 1110, 119, 119, 117, 117, 8, 118, 11
D18S5114.2, 1614.2, 1616, 1816, 1817, 2017, 2017, 1816, 17, 1816
D5S8181212131312, 1412, 1412, 1311, 12, 1311, 12
FGA20, 2220, 2221, 2621, 2624, 2824, 2822, 2322, 2322, 23
AmelogeninYYXXXXXX, YY
  • a Microsatellite analyses were performed using DNA extracted from plasma samples, except for subject ZM246M for whom only peripheral blood mononuclear cell DNA was available. Loci were amplified using an AmpFlSTR Identifiler kit (Applied Biosystems, Foster City, CA). This PCR amplification kit is a short tandem repeat multiplex assay that amplifies 15 tetranucleotide repeat loci and the amelogenin gender-determining marker in a single PCR amplification; all identifiable alleles per locus are shown.

  • b This sample was identified as an inadvertent mixture of plasma from two different individuals (ZM246F and ZM246M).

  • c Subject ZM246M is the chronically infected partner of subject ZM246F but not the individual who transmitted virus to subject ZM246F (see Fig. 7 and associated text for explanation).