TABLE 2.

Oligonucleotides used for real-time RT-PCR analysis

AmpliconForward primerReverse primer
NameSequence (5′ → 3′)NameSequence (5′ → 3′)
gRNART-REP-VSTTCTTTTGACAAAACATACGGTGAART-REP-RSCTAGGCAACTGGTTTGTAACATCTTT
mRNA-S1L-CS1 VSCCAACTCGAACTAAACTTTGGTAACCL-CS1 RSTCAATGGCATTACGACCAAAAC
mRNA-3a.1rt-3aVS2CGGACACCAACTCGAACTAAACTTACrt3a-RSATCAAGTTCGTCAAGTACAGCATCTAC
mRNA-ELdrt-VSCGTGGCTATATCTCTTCTTTTACTTTAACTAGmRNAE-RSCAATGCCCTAGGAAACGTCATAG
mRNA-MLdrt-VSCGTGGCTATATCTCTTCTTTTACTTTAACTAGmRNAM-RSGCATGCAATCACACACGCTAA
mRNA-NLdrt-VSCGTGGCTATATCTCTTCTTTTACTTTAACTAGN82RSTCTTCCGACCACGGGAATT
mRNA-7Ldrt-VSCGTGGCTATATCTCTTCTTTTACTTTAACTAG7(38)RSAAAACTGTAATAAATACAGCATGGAGGAA
mRNA-GFPLdrt-VSCGTGGCTATATCTCTTCTTTTACTTTAACTAGRT-GFP RSCGCCCTTGCTCACCATTTAG