TABLE 2.

Time course quantitative TIEM analysis of VP16 immunolabeling on intranuclear, cytoplasmic, and extracellular HSV-1 capsids at various times postinfection

Time (hpi)Label intensity% of capsids (n)a in:
NucleusCytoplasm (unenveloped)Cytoplasm (enveloped)Extra- cellular region
12Total100 (212)100 (34)100 (61)100 (58)
Negative63.75.9
Weak26.929.4
Moderate8.523.53.5
Dense0.941.210096.5
17Total100 (480)100 (68)100 (123)100 (103)
Negative84.844.1
Weak13.339.77.35.8
Moderate1.911.814.76.8
Dense4.478.087.4
24Total100 (470)100 (123)100 (76)100 (129)
Negative54.71.4
Weak24.526.04.02.3
Moderate12.823.6
Dense8.048.896.097.7
24 (plus BFA)Total100 (403)100 (144)100 (84)100 (0)
Negative74.439.628.6
Weak20.122.25.9
Moderate5.520.813.1
Dense17.452.4
  • a See Table 1, footnote a.