TABLE 1.

Effect of E1A on Vpr and p300 or p300(1945-2414) synergism on MMTV LTR in A204 cellsa

E1A-expressing plasmidProtein-expressing plasmid(s)Luciferase activity (RLU)b
Dex (−)Dex (+)
AbsentNone0.028 ± 0.00290.54 ± 0.037
Vpr0.018 ± 0.00445.25 ± 0.394
p3000.037 ± 0.00161.12 ± 0.265
p300 (1945-2414)0.031 ± 0.00650.58 ± 0.106
Vpr + p3000.025 ± 0.003815.92 ± 0.946
Vpr + p300 (1945-2414)0.029 ± 0.012314.05 ± 1.825
PresentNone0.027 ± 0.00550.28 ± 0.032
Vpr0.028 ± 0.00480.78 ± 0.295
p3000.031 ± 0.01380.32 ± 0.211
p300 (1945-2414)0.041 ± 0.00150.22 ± 0.141
Vpr + p3000.024 ± 0.00521.25 ± 0.231
Vpr + p300 (1945-2414)0.024 ± 0.01001.34 ± 0.381
  • a A204 cells were transfected with the indicated protein-expressing plasmids, MMTV-LTR-Luc, and pSV40-β-Gal in the absence or presence of the E1A-expressing plasmid, pCMV-12S-E1A. Cells were then exposed to 10−6 M dexamethasone [Dex (+)] or vehicle without dexamethasone [Dex (−)].

  • b Values are expressed as means ± SE.