TABLE 1.

Time course quantitative TIEM analysis of VP13/14 immunolabeling on intranuclear, cytoplasmic, and extracellular HSV-1 capsids at various times postinfection

Time (hpi)Label intensity% of capsids (n)a in:
NucleusCytoplasm (unenveloped)Cytoplasm (enveloped)Extra- cellular region
12Total100 (195)100 (31)100 (58)100 (36)
Negative91.5
Weak5.632.2
Moderate35.53.5
Dense32.296.5100
17Total100 (493)100 (39)100 (112)100 (76)
Negative96.848.86.313.2
Weak3.220.510.7
Moderate17.913.414.5
Dense12.869.672.3
24Total100 (344)100 (36)100 (61)100 (56)
Negative88.733.3
Weak11.327.819.6
Moderate38.94.9
Dense95.180.4
24 (plus BFA)Total100 (313)100 (37)100 (63)100 (0)
Negative93.048.722.2
Weak7.051.317.5
Moderate
Dense60.3
  • a The percentage values shown were derived by counting viral particles in 15 cell profiles for each tegument protein and for each time point. —, Complete absence. n = total number of viral particles.