TABLE 2.

Primers used for yeast gene disruption

GenePrimers
CDC55::KAN5′-CCTCATAAAATCTAGCCAACATATCGAGGTCAAACTGGAGAGGATATCAAGCTTGCCTCG-3′
5′-GAATTCAAGTTCAATTTAAATTTCAATTAAAACAGTAGTAGTATGTGGGGAAGATATGGGGTCGACACTGGATGGCGGC-3′
CDC55::HIS35′-GGAGTTTGCACAAGACAAG-3′
5′-GAAGTGATGAAAGAAGTCC-3′
CDC55::HYG5′-TCCTTGTGACTCCGCATAAACTAGATGATAAAGAGTACAAACAAGTCGCCCAGCTGAAGCTTCGTACGC-3′
5′-AACGGTAAGCATATTAAGATCAAATTAGTTGAGGCTGTAAATAAAAAGCATAGGCCACTAGTGGATCTG-3′
RTS1::KAN5′-CGGCCTACTTGATGCTCTACC-3′
5′-CTAAACTTCCTCACTTCTTCG-3′
PPM1::KAN5′-CTTATGCCATAAGAAGCAG-3′
5′-TGGAACAATGCCCTGCATC-3′