TABLE 1.

Yeast strains used in the study

Yeast strainGenotypeReference or source
W303-1AMATa ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 can1-10035
DEY3aMATa pph21Δ::LEU2 pph22Δ1::HIS3 pph3Δ1::LYS2 lys2-951 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 ssd1-d1 {YCpDE8 PPH22} W30311
ADR496aMATa ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 can1-100 cdc55Δ::HIS333
ΔTPD3aMATα ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 can1-100 tpd3Δ::LEU54
BY4741bMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0Research Genetics
ΔCDC55bMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 cdc55Δ::HIS3This study
ΔPPH3bMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 pph3Δ::KANResearch Genetics
ΔPPT1bMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ppt1Δ::KANResearch Genetics
ΔPPZ1bMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ppzΔ::KANResearch Genetics
ΔSIT4bMATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sit4Δ::KANResearch Genetics
YDP001bSIT4ΔCDC55)MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 sit4Δ::KAN cdc55Δ::HIS3This study
YDP002aCDC55ΔTPD3)MATa ura3-1 leu2-3,112 trp1-1 his3-11,15 ade2-1 can1-100 cdc55::HIS3 tpd3::LEU W303This study
YDP003aRTS1)MATa pph21Δ::LEU2 pph22Δ1::HIS3 pph3Δ1::LYS2 lys2-951 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 ssd1-d1 rts1::KAN {YCpDE8 PPH22} W303This study
YDP004aCDC55)MATa pph21Δ::LEU2 pph22Δ1::HIS3 pph3Δ1::LYS2 lys2-951 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 ssd1-d1 cdc55::KAN {YCpDE8 PPH22} W303This study
YDP005aPPM1)MATa pph21Δ::LEU2 pph22Δ1::HIS3 pph3Δ1::LYS2 lys2-951 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 ssd1-d11 ppm1::KAN {YCpDE8 PPH22} W303This study
YDP006aPPM1ΔCDC55)MATa pph21Δ::LEU2 pph22Δ1::HIS3 pph3Δ1::LYS2 lys2-951 ade2-1 ura3-1 his3-11 trp1-1 leu2-3,112 ssd1-d1 ppm1::KAN cdc55::HYG {YCpDE8 PPH22} W303This study
  • a Isogenic with W303-1A.

  • b Isogenic with BY4741.