TABLE 1.

Frequencies of viral genome-positive and reactivating splenocytes at day 16 postinfection

VirusTotal no. of splenocytes recoveredReciprocal frequency of viral genome-positive cellsaGenome frequency relative to wt virus frequencyEstimated no. of viral genome-positive cellsbReciprocal frequency of reactivating cellsaReactivation frequency relative to wt virus frequencyEstimated no. of reactivating cellsbReactivation efficiency (%)c
wt ΜHV684.12 × 1071691.0243,7873,1151.013,2265.4
M2.Stop(2)2.64 × 1076,4230.03d4,110d>1 × 106<0.01<25d∼0.5
M2.P18.97 × 1074200.40213,57160,0420.051,494d0.7
M2.P1.MR1.12 × 1082150.79520,9304,0460.7727,6825.3
M2.P25.24 × 1071830.92286,33910,0700.315,2031.8
M2.P3/46.82 × 1072230.75305,8295,7450.5411,8713.9
M2.P56.30 × 1071720.98366,2796,0460.5210,4202.8
M2.P68.37 × 1071301.30643,8461,7771.7547,1027.3
M2.P73.66 × 1073900.4393,84615,1450.212,4172.6
M2.P84.18 × 1072,1230.08d19,689d85,7250.04488d2.5
M2.P8.MR6.08 × 1071780.95341,5737,8760.407,7202.3
M2.P95.75 × 1071,8980.09d30,295d56,1250.061,024d3.4
M2.P9.MR6.80 × 1071601.06425,0003,1261.021,5295.1
M2.P8/96.03 × 1071,9010.09d31,720d149,4160.02404d1.3
M2.P8/9.MR1.04 × 1081611.05645,9634,4200.7023,5293.6
M2.P3/4/74.33 × 1073610.47119,9449,5690.334,5253.8
M2.Y120/129F8.97 × 1074920.34182,31715,7000.205,7163.1
M2.Y120/129F.MR5.62 × 1072120.80265,0944,4210.7012,7124.8
M2.Y120/129D5.24 × 1072,2030.08d23,786d150,3770.02348d1.5
M2.Y120/129D.MR7.95 × 1071451.17548,2762,7851.1128,5465.2
M2.Y129F/P72.03 × 1073,3060.05d6,140d231,4890.0188d1.4
M2.Y129F/P7.MR6.72 × 1071900.89353,6842,2971.3629,2558.4
  • a Determined from the mean of at least two independent experiments, with splenocytes pooled from five mice per experimental group.

  • b Estimated number per spleen, derived from the experimental frequency data and the total number of splenocytes recovered.

  • c Percentage of viral genome-positive splenocytes that reactivate virus.

  • d The data of significance are indicated in bold.