TABLE 3.

Double deletion of CDC55 and TPD3 does not rescue cells from E4orf4-induced growth inhibition more effectively than deletion of TPD3 alonea

Yeast straintd ratio
Wild type (W303)1.58 ± 0.08
Δtpd31.11 ± 0.12
Δcdc55 Δtpd31.13 ± 0.09
  • a Cells were grown in 2% glucose medium to log phase. Cells were then washed and grown in 2% raffinose medium for 2 h. Cells were then counted and diluted to equal number of cells or were diluted to an optical density at 600 nm (OD600) of 0.05. The growth of the cells was monitored by counting cells using a hemacytometer or by measuring the OD600. To accurately measure the OD, cells were diluted at some point so that the OD600 reading would be below 1.0. The doubling times (td) of the cells were calculated using the cell numbers and times from the exponential phase for regression analysis. The ratios of the td for cells expressing Eforf4 over the td for cells with vector only are reported as mean ± standard deviation.