TABLE 2.

Phenotypic analyses of mutant PA proteins

Mutation (amino acid)CAT activityaProteinbViral rescuec
WT++++
W406A+++
E410A+++
L425A+++NT+
K461A+++NT+
G502A
S509A+++NT+
H510A+
D514A+++NT+
E524A
S526A+++NT+
K539A+++
V542A+++NT+
L563A+++NT+
R583A+++NT+
G622A++++
S624A++++
R638A++++
Q654A++++
E656A++++
G657A+++
F568A+++NT+
E692A++NT+
C693A+++NT+
N696A+++NT+
P698A+++NT+
N708A+++NT+
Q654A(Δ655-658)
C693A(Δ694-716)
  • a +++, 10 to 100%; ++, 1 to 10%; +, <1%; —, not detectable.

  • b ✓, expressed to wild-type levels; NT, not tested.

  • c +, yes; —, no viral rescue was achieved.