TABLE 1.

Surface expression of wt F protein and cytoplasmic tail-truncated F proteinsa

Protein expressedSurface expression efficiency from:
Transfected DNAbRecombinant virusc
% Positive cellsRelative MFI% Positive cellsRelative MFI
F58.121.0099.121.00
FΔ256.040.9598.960.89
FΔ461.911.1097.481.07
FΔ661.320.8988.270.85
FΔ863.571.0094.491.14
FΔ1059.451.0297.080.91
FΔ1267.141.0297.980.92
FΔ1458.620.9798.550.86
FΔ1653.440.9298.650.95
FΔ1860.620.9898.430.87
FΔ2048.280.6081.620.22
  • a Surface expression efficiency was determined by flow cytometry. CV-1 cells were bound with MAb F1a and an FITC-conjugated secondary antibody. MFI, mean fluorescence intensity.

  • b Expression of F protein by using the vaccinia virus T7 expression system.

  • c Expression of F protein from rSV5-infected cells.