TABLE 1.

Results of FLIM analysis

(Co)transfection (Fig. 5 panel)τφ ± SD (ns)aτM ± SD (ns)bRes (%)c
2B-ECFP + 2B-EYFP (A)d2.42 ± 0.132.99 ± 0.131.7
2B-ECFP + 2B-EYFP (B)2.13 ± 0.192.66 ± 0.161.9
2B-ECFP + EYFP (C)2.53 ± 0.113.03 ± 0.101.7
EYFP-ECFP fusion (D)1.95 ± 0.052.60 ± 0.031.5
  • a Fluorescence lifetime calculated from the phase shift Δφ (τφ = 1/ωtan Δφ). SD, standard deviation in the τφ image corrected for noise in the fit procedure (17).

  • b Fluorescence lifetime calculated from the modulation (M) with τM = (1/ω) (M−2 − 1)1/2. SD, standard deviation in the τM image corrected for noise in the fit procedure (17).

  • c The quality of the data is indicated by the low average difference between calculated (lc) and observed (lo) fluorescence intensity in each phase image: Res2 = (lolc)2/lc2 (17).

  • d Note that the τφ and the τM values shown in Fig. 5A differ from those in Fig. 5B, because in Fig. 5A an overview of the cell population is shown (of which only a limited number exhibit a reduced fluorescence lifetime), whereas Fig. 5B shows only two cells (of which one exhibits a major reduction in fluorescence lifetime, whereas the other one exhibits a minor decrease in fluorescence lifetime).