TABLE 1.

Reconstitution efficiencies of UL74 null mutants with or without additional deletions

Mutant BACDeletion(s)No. of reconstitutions/no. of transfections
TB40-BAC4-ΔUL74nt1-37UL749/9
TB40-BAC4-ΔUL74nt1-37-ΔUL128-131kanUL74, UL128-131A0/3
TB40-BAC1-ΔUL74kanUL74, UL1280/2
TB40-BAC4-ΔUL74nt1-37-UL128insA332UL74, UL1280/6
TB40-BAC4-ΔUL74nt1-37-ΔUL131kanUL74, UL131A1/9
TB40/BAC4-UL131stop-ΔgO-kanUL74, UL131A0/6
pHB5-ΔUL74nt1-42 (AD169 based)UL74, UL131A0/12