TABLE 1.

Half-life measurements for preintegration latency in infected resting CD4+ T cellsa

Expt no.MOIbAcid treatmentcDecay rated (days−1)Half-lifee (days)R2 of regression
12No0.591.20.99
25No0.780.890.86
37No1.00.690.98
410Yes0.820.840.88
510Yes0.780.890.98
620Yes0.960.720.89
  • a Highly purified resting CD4+ T cells were infected with NL4-1-GFP and incubated for various times up to 3 days before activation and analysis of induced GFP expression.

  • b MOI, multiplicity of infection.

  • c In some experiments, infected cells were washed briefly at an acidic pH to promote direct fusion of the virus with the plasma membrane.

  • d Decay rates were determined by modeling the decay data from the first 3 days postinfection as an exponential process.

  • e The average half-life for the six experiments was 0.9 ± 0.2 days.