TABLE 3.

Biophysical characterization of JR-FL gp41 core mutantsa

Mutantθ222 (degrees/cm2/dmol)Tm (°C)Mobs/Mcalc
N36(L6)C3432,500783.1
I559G32,300462.9
I559P25,60034b
L566V32,100723.1
T569P29,400443.0
  • a All CD scans and melts were performed with 10 μM peptide solutions in PBS (pH 7.0). Tm was estimated from the thermal dependence of θ222. Sedimentation equilibrium results are reported as a ratio of the experimentally determined molecular weight to the calculated molecular weight for a monomer (M obs/M calc).

  • b —, Aggregated, as determined by sedimentation equilibrium analysis.