TABLE 2.

Expression of FcγR homologs by HCMV ts9- and pHB5-derived deletion mutantsa

MutantDeletionExpression of:
gp34gp68
ts9bΔUS1-15++
HB5-ΔUL1-1411660-21440c++
HB5-ΔUL14-2020883-26433++
HB5-ΔUL40-4253401-55537++
HB5-ΔUL118-120168430-169579+
HB5-ΔUL128-132175840-178619++
HB5-ΔIRL179150-192329++
HB5-ΔTRL10-14/IRL8051-11150 and 179150-192329+
  • a MRC5 cells were infected at an MOI of 3 for 72 h with the HCMV mutant indicated. Cells were metabolically labeled with [35S]methionine, and proteins from lysates were precipitated with Fc fragments and protein A Sepharose and separated by SDS11.5 to 15% PAGE. + indicates presence of the FcBP; − indicates absence of the FcBP.

  • b See reference 46.

  • c Ranges represent nucleotide positions.