TABLE 1.

Ig binding specifity of the Fc-binding proteins gp34 and gp68a

SpeciesIg subtypeIg binding by:
gp34gp68
HumanIgG1++++
HumanIgG2++++
HumanIgG3++++
HumanIgG4++++
HumanIgM
HumanIgA
RabbitIgG++
MouseIgG2b
RatIgG1+
BovineIgG
GoatIgG
  • a MRC5 cells were infected at an MOI of 3 for 72 h with HCMV AD169 before cells were metabolically labeled with [35S]methionine and proteins from lysates were precipitated with indicated antibodies and protein A Sepharose. Precipitated proteins were separated by SDS-11.5 to 15% PAGE. ++ indicates strong binding; + indicates weak binding; − indicates no binding.