TABLE 1.

Class I and class II binding and degradation properties of US2 mutantsa

US2 or US2 mutant (residues)BindingDegradation
Class IClass IIClass IClass II
US2 wild type+++++++++++++++
1-186+++++++
1-160+++++++
1-150++++
1-140+++
1-130
1-120
1-110
Kb21-199+++++++++++++++
Kb28-199+++++++++++++
Kb40-199++++++
US2/3-hr1 (47-66)+++++++
US2/3-hr2 (94-111)+++
US2 Δhr3 (132-135)+
  • a The binding and degradation of class I and class II molecules by wild-type and mutant forms of US2 was quantified by phosphorimager analysis of the gels shown in Fig. 3, 4, and 5.