TABLE 1.

MAb recognition of various E2 glycoprotein forms

MAbaEpitopeb (aa)MAb recognition of the indicated H77c E2 antigenc
FLE1E2, intracellularE2660 IntracellularSecretedVLPI-EM
NDNDNDND
7/59384-395 (HVR-1)+++++++++++++++++++
6/82a384-395 (HVR-1)+++++++++++
6/16384-395 (HVR-1)+++++++++++++
9/86aConf. (HVR-1)+++++++++++++++++++
9/27396-407 (HVR-1)+++++++++++++++++++++++++
3/11412-423+++++++++++++++++++++
AP33412-423+++++++++++++++++++++
2/69a432-443+++++++++++++++++++++++++
1/39436-443+++++++++++++++++++++++++++
11/20c436-447++++++++++++++++++++++++++
7/16b436-447++++++++++++++++++++++++++
AP320464-471++++++++++++++++++
AP436464-475+++++++++
AP109464-475++++++++
6/41a480-493++++++++++++++++++++++++
2/64a524-531+++++++++++++++++++++++
ALP42524-535++++++
9/75528-535++++++++++++++++++++++++++
6/53544-551+++++++++++++++++++++++++++
AP266644-651+++++++++++++++++++
ALP98644-651++++++++++++++++++++
ALP11644-651++++++++++/−++++++
ALP1648-659+++++++++++++++++++++
H53Conf.+++++++++++++++
H60Conf.++++++++++++++
  • a MAbs of particular interest that are discussed in the text are boldfaced.

  • b Conf., conformational.

  • c The FL E1E2 complex and E2660 were expressed in COS-7 cells, whereas VLPs were generated in Sf cells infected with rbac-B45. All ligands were tested by GNA-capture ELISA as described in Materials and Methods. In addition, VLPs were also analyzed by immunogold-EM (I-EM). +, ++, and +++, optical density readings between 5 and 10%, 10 and 30%, and 30 and 60%, respectively, above background values. N, native. D, denatured.