TABLE 1.

Primers used to amplify and sequence the P2 and P3 coding regions

PCR fragmentOrientationSequence5′ Position (nucleotide)
Product 1Forward5′-AAACTCAGGTGCCAAGCGGTATGC-3′2694
Reverse5′-CCTCTTAGTGAGCACGACCACAGCA-3′3414
Product 2Forward5′-GAACGTGAACTTCCAACCCAGCG-3′3231
Reverse5′-ATTACGGAATCCCTATGGCTGAACG-3′4016
Product 3Forward5′-GGTGAGGAACCACGATGACCTGATC-3′3915
Reverse5′-CGTCTCCTTGTTCTGCCAAATGGT-3′4602
Product 4Forward5′-TTAATTGGAAGGTCGCTTGC-3′4465
Reverse5′-CACATTTGTGTCAGTGGCTG-3′5238
Product 5Forward5′-TGCTCAAATCGGTAGACAGTGAGGC-3′5105
Reverse5′-CCATCAGGGCTTCTCAGCAGCA-3′5862
Product 6Forward5′-CTTAGCCAAGGAGGAAGTGG-3′5631
Reverse5′-AGAAGGTAGCGAAAGGAAAG-3′6410
Product 7Forward5′-CGGTTACCCATATGTTGCAC-3′6261
Reverse5′-CTGAAGCTGGTAAGGGTTAC-3′6947
Product 8Forward5′-AAAGTGTACAAAGGGATTGACTTGG-3′6847
Reverse5′-ATTCGGTGCGGAAAAAAAAAAA-3′7392