Comparison of the effects of select mutations in gp120JR-CSF on the binding of MAbs 2G12 and IgG1 b12
| Mutationa | Affinity of antibody for indicated mutant relative to wild type (%) | |
|---|---|---|
| 2G12 | IgG1 b12 | |
| N295A | 2 | 80 |
| N295Q | 4 | 261 |
| N332A | 3 | 75 |
| N339A | 8 | 30 |
| N339Q | 91 | 567 |
| N386A | 21 | 23 |
| N392A | 4 | 4 |
| N392Q | 6 | 142 |
| S334T | 10 | 6 |
| L416A | 2 | 2 |
| F353A | 15 | 29 |
| K421A | 19 | 18 |
| K404A | 32 | 101 |
| S406A | 33 | 60 |
| T408A | 40 | 96 |
| S365A | 1,173 | 667 |
| G458A | 349 | 857 |
↵a Amino acid numbering is based on the HxB2 sequence.