TABLE 1.

The V3-loop is the major viral determinant of cell surface GAG-mediated effects on HIV-1 infection

EnvelopeInfectious titer (FFU/ml)a (ratiob)
HXBADAJRFLV3(ADA/JRFL)c
CHO-K1(5.3 ± 1.1) × 106(8.2 ± 0.4) × 105(1.6 ± 0.4) × 106(2.8 ± 0.6) × 106
pgsA-745(5.6 ± 0.5) × 105 (9.5)(4.2 ± 0.6) × 106 (0.19)(6.8 ± 0.7) × 106 (0.24)(7.3 ± 1.7) × 106 (0.38)
  • a Infectious titers of NL4-3-derived viruses containing the indicated envelope genes were measured by using CHO-K1- and pgsA745-derived target cells, and foci of infection were enumerated after immunofluorescence as described in Materials and Methods. FFU, focus-forming units.

  • b Titer on CHO-K1-derived target cells/titer on pgsA745-derived target cells.

  • c V3(ADA/JRFL) is a chimeric envelope containing V3-loop sequences of ADA/JRFL in in an HXB background.